2009-03-30

centromeric function of Ku70 was not observed in 14 other grasshopper and locust species, or in the mouse, thus suggesting that it is an autapomorphy in E. plorans. Keywords Autapomorphy.Centromere. Eyprepocnemisplorans.Geneknockdown. Immunofluorescence.Kinetochore.Ku70.Ku80. Microtubules.Orthoptera.RNAi.Spindleassembly checkpoint Abbreviations

It is a heterodimeric protein made up of Ku70 and Ku80 [97] . We (unpublished data) and others [43] had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation. There was no correlation between the transcript levels of Ku80 and LUC luminescence derived from T‐DNA stable transformation in KD‐OsKu80 rice calli (Figs 1b, 2d,e). It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994). Biochemical analyses of the Ku70-Ku80 het-erodimer protein demonstrated that it bound in a sequence-nonspecific fashion to virtually all double-stranded DNA ends including 5 -or3-protruding ends, blunt ends (32), and duplex DNA ending in stem–loop structures (33). The DNA binding activity of the Ku70-Ku80 heterodimer is thought to function by LSE is an autoimmune disease, whereby the immune system mistakenly attacks healthy tissue, resulting in red rash, as a response of inflammation.

Despite recent advances in our understanding of the function of long noncoding RNAs (lncRNAs), their roles and functions in DNA repair pathways remain poorly understood. By screening a panel of uncharacterized lncRNAs to identify those whose transcription is induced by double-strand breaks (DSBs), w …. In eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the “classical” non-homologous end joining (C-NHEJ) pathway, the main DNA double-strand break (DSB) repair pathway in mammals. Ku, the heterodimer of Ku70 and Ku80, plays an essential role in the DNA double-strand break (DSB) repair pathway, i.e., non-homologous end-joining (NHEJ). Two isoforms of Ku80 encoded by the same genes, namely, Ku80 and KARP-1 are expressed and function in primate cells, but not in rodent cells.

Ku70 is one component of a protein complex, Ku70 and Ku80, that functions as a heterodimer to bind DNA double-strand breaks and activates DNA-dependent protein kinase. Our previous study with Ku70−/− and Ku80−/− mice, and cell lines has shown that Ku70- and Ku80-deficiency compromises the ability of cells to repair DNA double-strand breaks, increases radiosensitivity of cells, and

Next, the broken DNA is ligated through a complex consisting of DNA ligase IV (Lig4), XRCC4, and Cernunnos/XLF (1). Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36). Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background .

The information may be useful in examining the function of the Ku complex, as well Pull-down analysis with cytosolic proteins identified Ku70 and Ku80 as the

Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background . XRCC4, is a protein of unknown function. The Ku70 protein is an additional component of DNA-PK; Ku70 forms a het-erodimer with Ku80 to generate the DNA end-binding com-ponent of the enzyme. To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression. We find Ku70 is one component of a protein complex, Ku70 and Ku80, that functions as a heterodimer to bind DNA double-strand breaks and activates DNA-dependent protein kinase.

Interaction of Ku with DNA is central for the functions of Ku. 2001-03-01 · Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Ku80 is a protein that, in humans, is encoded by the XRCC5 gene. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair. We developed a novel assay to measure DNA binding and release kinetics using differences in Förster resonance energy transfer (FRET) of the ECFP-Ku70/EYFP-Ku80 heterodimer in soluble and DNA end bound states. Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks.
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We developed a novel assay to measure DNA binding and release kinetics using differences in Förster resonance energy transfer (FRET) of the ECFP-Ku70/EYFP-Ku80 heterodimer in soluble and DNA end bound states. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection.

Results. Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information. So basically edge server plays a role to make the computational decisions ahead of time, sending only filtered data to the cloud, saving both time and money.
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The Ku70–Ku80 heterodimer of other species binds to the termini of double‐stranded DNA as the initial step in DSB repair. To determine whether the AtKu70–AtKu80 heterodimer also binds to DNA fragments, we performed EMSA analysis with the recombinant proteins produced in E. coli .

Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase.